Protein Analysis of Babesia caballi Merozoites by Two-Dimensional Polyacrylamide Gel Electrophoresis and Western Blotting
نویسندگان
چکیده
منابع مشابه
Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis.
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. I...
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A preparative isoelectric focusing (IEF) method was applied to separate skim milk proteins using the Rotofor device in a pH 3-10 gradient containing 4 M urealI % triton X-I 00. Each ofthe 20 fractions obtainedfrom the Rotofor device was then analyzed by polyacrylamide gel electrophoresis (PAGE). Both ureaPAGE and SDS-PAGE were used to separate purified caseins and skim milk resulting in compara...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2000
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.62.323